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Ridom GmbH spa server – spatypes
Spa Server – Spatypes, supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spa-server/spa+server+++spatypes/pm38697483-58-13-14
Average 90 stars, based on 1 article reviews
spa server – spatypes - by Bioz Stars, 2026-10
90/100 stars

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Polymerase Chain Reaction:

Article Title: Toxic bovine mastitis caused by Staphylococcus aureus in twin cows.
Article Snippet: In this report, we describe two cases of bovine toxic mastitis associated with S. aureus and we provide DNA microarray based characterization data of the strain causing the disease.. Both cows had recently calved and suffered from anorexia, pyrexia, and an elevated heart rate.. In both animals, at least one mammary gland was swollen, hardened, sensitive to touch, and produced brownish or bloody secretions.

Article Title: Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms
Article Snippet: The spa types were determined using the spa -server ( http://spa.ridom.de/ ;) ( ).

Article Title: East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus.
Article Snippet: Accepted Manuscript East and West African milk products are reservoirs for human and livestockassociated Staphylococcus aureus Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A. Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara PII: S0740-0020(16)30762-6 DOI: 10.1016/j.fm.2017.01.017 Reference: YFMIC 2726 To appear in: Food Microbiology Received Date: 16 September 2016 Revised Date: 12 January 2017 Accepted Date: 29 January 2017 Please cite this article as: Jans, C., Merz, A., Johler, S., Younan, M., Tanner, S.A., Kaindi, D.W.M., Wangoh, J., Bonfoh, B., Meile, L., Tasara, T., East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus, Food Microbiology (2017), doi: 10.1016/ j.fm.2017.01.017.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: An in-92 house analysis pipeline has been developed to analyse MRSA genomes for mecA, mecC, nuc, ccr 93 genes, spa-type, MLST, dru types, PVL and ACME (the arginine catabolic mobile element). spa-94 types were called from the assembled contigs by comparison to the published types on the Spa-95 server (http://spa.ridom.de/index.shtml).

Article Title: <p>In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of <em>Staphylococcus aureus</em> Isolates from Bovine Mastitis in the Liaoning Province of China</p>
Article Snippet: The PCR amplicons were purified using the MiniBEST DNA Fragment Purification Kit (TaKaRa, Dalian, China) and then sequenced; the spa types were determined using the spa-server (http://spa.ridom.de/).17 Antimicrobial Susceptibility Test The antibiotic resistance phenotype was determined using the microdilution method in MH(B) broth (AoBox) according to the Clinical Laboratory and Standards Institute (CLSI) guidelines.18 For each isolate, five colonies from the agar medium were inoculated into the broth medium in order to reach adequate growth; the inoculums were then transferred into tubes containing sterile 0.85% saline (Solarbio, Beijing, China), and the inoculums were mixed with the saline.

Article Title: Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers.
Article Snippet: The amplified spa gene fragment was purified, sequenced and spa types were assigned using the Spa-server (Ridom GmbH, Würzburg, Germany) [27].

Article Title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals 31 and the community.. As the rapid spread and wide distribution of antimicrobial resistance (such as 32 MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat.. Thus, 33 early identification and genotyping are essential for further therapeutic treatment and the control of 34 rapid expansion of MRSA.

Purification:

Article Title: Toxic bovine mastitis caused by Staphylococcus aureus in twin cows.
Article Snippet: In this report, we describe two cases of bovine toxic mastitis associated with S. aureus and we provide DNA microarray based characterization data of the strain causing the disease.. Both cows had recently calved and suffered from anorexia, pyrexia, and an elevated heart rate.. In both animals, at least one mammary gland was swollen, hardened, sensitive to touch, and produced brownish or bloody secretions.

Article Title: Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms
Article Snippet: The spa types were determined using the spa -server ( http://spa.ridom.de/ ;) ( ).

Article Title: East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus.
Article Snippet: Accepted Manuscript East and West African milk products are reservoirs for human and livestockassociated Staphylococcus aureus Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A. Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara PII: S0740-0020(16)30762-6 DOI: 10.1016/j.fm.2017.01.017 Reference: YFMIC 2726 To appear in: Food Microbiology Received Date: 16 September 2016 Revised Date: 12 January 2017 Accepted Date: 29 January 2017 Please cite this article as: Jans, C., Merz, A., Johler, S., Younan, M., Tanner, S.A., Kaindi, D.W.M., Wangoh, J., Bonfoh, B., Meile, L., Tasara, T., East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus, Food Microbiology (2017), doi: 10.1016/ j.fm.2017.01.017.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: An in-92 house analysis pipeline has been developed to analyse MRSA genomes for mecA, mecC, nuc, ccr 93 genes, spa-type, MLST, dru types, PVL and ACME (the arginine catabolic mobile element). spa-94 types were called from the assembled contigs by comparison to the published types on the Spa-95 server (http://spa.ridom.de/index.shtml).

Article Title: <p>In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of <em>Staphylococcus aureus</em> Isolates from Bovine Mastitis in the Liaoning Province of China</p>
Article Snippet: The PCR amplicons were purified using the MiniBEST DNA Fragment Purification Kit (TaKaRa, Dalian, China) and then sequenced; the spa types were determined using the spa-server (http://spa.ridom.de/).17 Antimicrobial Susceptibility Test The antibiotic resistance phenotype was determined using the microdilution method in MH(B) broth (AoBox) according to the Clinical Laboratory and Standards Institute (CLSI) guidelines.18 For each isolate, five colonies from the agar medium were inoculated into the broth medium in order to reach adequate growth; the inoculums were then transferred into tubes containing sterile 0.85% saline (Solarbio, Beijing, China), and the inoculums were mixed with the saline.

Article Title: Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers.
Article Snippet: The amplified spa gene fragment was purified, sequenced and spa types were assigned using the Spa-server (Ridom GmbH, Würzburg, Germany) [27].

Article Title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals 31 and the community.. As the rapid spread and wide distribution of antimicrobial resistance (such as 32 MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat.. Thus, 33 early identification and genotyping are essential for further therapeutic treatment and the control of 34 rapid expansion of MRSA.

Sterility:

Article Title: Toxic bovine mastitis caused by Staphylococcus aureus in twin cows.
Article Snippet: In this report, we describe two cases of bovine toxic mastitis associated with S. aureus and we provide DNA microarray based characterization data of the strain causing the disease.. Both cows had recently calved and suffered from anorexia, pyrexia, and an elevated heart rate.. In both animals, at least one mammary gland was swollen, hardened, sensitive to touch, and produced brownish or bloody secretions.

Article Title: Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms
Article Snippet: The spa types were determined using the spa -server ( http://spa.ridom.de/ ;) ( ).

Article Title: East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus.
Article Snippet: Accepted Manuscript East and West African milk products are reservoirs for human and livestockassociated Staphylococcus aureus Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A. Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara PII: S0740-0020(16)30762-6 DOI: 10.1016/j.fm.2017.01.017 Reference: YFMIC 2726 To appear in: Food Microbiology Received Date: 16 September 2016 Revised Date: 12 January 2017 Accepted Date: 29 January 2017 Please cite this article as: Jans, C., Merz, A., Johler, S., Younan, M., Tanner, S.A., Kaindi, D.W.M., Wangoh, J., Bonfoh, B., Meile, L., Tasara, T., East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus, Food Microbiology (2017), doi: 10.1016/ j.fm.2017.01.017.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: An in-92 house analysis pipeline has been developed to analyse MRSA genomes for mecA, mecC, nuc, ccr 93 genes, spa-type, MLST, dru types, PVL and ACME (the arginine catabolic mobile element). spa-94 types were called from the assembled contigs by comparison to the published types on the Spa-95 server (http://spa.ridom.de/index.shtml).

Article Title: <p>In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of <em>Staphylococcus aureus</em> Isolates from Bovine Mastitis in the Liaoning Province of China</p>
Article Snippet: The PCR amplicons were purified using the MiniBEST DNA Fragment Purification Kit (TaKaRa, Dalian, China) and then sequenced; the spa types were determined using the spa-server (http://spa.ridom.de/).17 Antimicrobial Susceptibility Test The antibiotic resistance phenotype was determined using the microdilution method in MH(B) broth (AoBox) according to the Clinical Laboratory and Standards Institute (CLSI) guidelines.18 For each isolate, five colonies from the agar medium were inoculated into the broth medium in order to reach adequate growth; the inoculums were then transferred into tubes containing sterile 0.85% saline (Solarbio, Beijing, China), and the inoculums were mixed with the saline.

Article Title: Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers.
Article Snippet: The amplified spa gene fragment was purified, sequenced and spa types were assigned using the Spa-server (Ridom GmbH, Würzburg, Germany) [27].

Article Title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals 31 and the community.. As the rapid spread and wide distribution of antimicrobial resistance (such as 32 MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat.. Thus, 33 early identification and genotyping are essential for further therapeutic treatment and the control of 34 rapid expansion of MRSA.

Saline:

Article Title: Toxic bovine mastitis caused by Staphylococcus aureus in twin cows.
Article Snippet: In this report, we describe two cases of bovine toxic mastitis associated with S. aureus and we provide DNA microarray based characterization data of the strain causing the disease.. Both cows had recently calved and suffered from anorexia, pyrexia, and an elevated heart rate.. In both animals, at least one mammary gland was swollen, hardened, sensitive to touch, and produced brownish or bloody secretions.

Article Title: Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms
Article Snippet: The spa types were determined using the spa -server ( http://spa.ridom.de/ ;) ( ).

Article Title: East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus.
Article Snippet: Accepted Manuscript East and West African milk products are reservoirs for human and livestockassociated Staphylococcus aureus Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A. Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara PII: S0740-0020(16)30762-6 DOI: 10.1016/j.fm.2017.01.017 Reference: YFMIC 2726 To appear in: Food Microbiology Received Date: 16 September 2016 Revised Date: 12 January 2017 Accepted Date: 29 January 2017 Please cite this article as: Jans, C., Merz, A., Johler, S., Younan, M., Tanner, S.A., Kaindi, D.W.M., Wangoh, J., Bonfoh, B., Meile, L., Tasara, T., East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus, Food Microbiology (2017), doi: 10.1016/ j.fm.2017.01.017.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: An in-92 house analysis pipeline has been developed to analyse MRSA genomes for mecA, mecC, nuc, ccr 93 genes, spa-type, MLST, dru types, PVL and ACME (the arginine catabolic mobile element). spa-94 types were called from the assembled contigs by comparison to the published types on the Spa-95 server (http://spa.ridom.de/index.shtml).

Article Title: <p>In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of <em>Staphylococcus aureus</em> Isolates from Bovine Mastitis in the Liaoning Province of China</p>
Article Snippet: The PCR amplicons were purified using the MiniBEST DNA Fragment Purification Kit (TaKaRa, Dalian, China) and then sequenced; the spa types were determined using the spa-server (http://spa.ridom.de/).17 Antimicrobial Susceptibility Test The antibiotic resistance phenotype was determined using the microdilution method in MH(B) broth (AoBox) according to the Clinical Laboratory and Standards Institute (CLSI) guidelines.18 For each isolate, five colonies from the agar medium were inoculated into the broth medium in order to reach adequate growth; the inoculums were then transferred into tubes containing sterile 0.85% saline (Solarbio, Beijing, China), and the inoculums were mixed with the saline.

Article Title: Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers.
Article Snippet: The amplified spa gene fragment was purified, sequenced and spa types were assigned using the Spa-server (Ridom GmbH, Würzburg, Germany) [27].

Article Title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals 31 and the community.. As the rapid spread and wide distribution of antimicrobial resistance (such as 32 MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat.. Thus, 33 early identification and genotyping are essential for further therapeutic treatment and the control of 34 rapid expansion of MRSA.

Amplification:

Article Title: Toxic bovine mastitis caused by Staphylococcus aureus in twin cows.
Article Snippet: In this report, we describe two cases of bovine toxic mastitis associated with S. aureus and we provide DNA microarray based characterization data of the strain causing the disease.. Both cows had recently calved and suffered from anorexia, pyrexia, and an elevated heart rate.. In both animals, at least one mammary gland was swollen, hardened, sensitive to touch, and produced brownish or bloody secretions.

Article Title: Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms
Article Snippet: The spa types were determined using the spa -server ( http://spa.ridom.de/ ;) ( ).

Article Title: East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus.
Article Snippet: Accepted Manuscript East and West African milk products are reservoirs for human and livestockassociated Staphylococcus aureus Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A. Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara PII: S0740-0020(16)30762-6 DOI: 10.1016/j.fm.2017.01.017 Reference: YFMIC 2726 To appear in: Food Microbiology Received Date: 16 September 2016 Revised Date: 12 January 2017 Accepted Date: 29 January 2017 Please cite this article as: Jans, C., Merz, A., Johler, S., Younan, M., Tanner, S.A., Kaindi, D.W.M., Wangoh, J., Bonfoh, B., Meile, L., Tasara, T., East and West African milk products are reservoirs for human and livestock-associated Staphylococcus aureus, Food Microbiology (2017), doi: 10.1016/ j.fm.2017.01.017.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: An in-92 house analysis pipeline has been developed to analyse MRSA genomes for mecA, mecC, nuc, ccr 93 genes, spa-type, MLST, dru types, PVL and ACME (the arginine catabolic mobile element). spa-94 types were called from the assembled contigs by comparison to the published types on the Spa-95 server (http://spa.ridom.de/index.shtml).

Article Title: <p>In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of <em>Staphylococcus aureus</em> Isolates from Bovine Mastitis in the Liaoning Province of China</p>
Article Snippet: The PCR amplicons were purified using the MiniBEST DNA Fragment Purification Kit (TaKaRa, Dalian, China) and then sequenced; the spa types were determined using the spa-server (http://spa.ridom.de/).17 Antimicrobial Susceptibility Test The antibiotic resistance phenotype was determined using the microdilution method in MH(B) broth (AoBox) according to the Clinical Laboratory and Standards Institute (CLSI) guidelines.18 For each isolate, five colonies from the agar medium were inoculated into the broth medium in order to reach adequate growth; the inoculums were then transferred into tubes containing sterile 0.85% saline (Solarbio, Beijing, China), and the inoculums were mixed with the saline.

Article Title: Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers.
Article Snippet: The amplified spa gene fragment was purified, sequenced and spa types were assigned using the Spa-server (Ridom GmbH, Würzburg, Germany) [27].

Article Title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals 31 and the community.. As the rapid spread and wide distribution of antimicrobial resistance (such as 32 MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat.. Thus, 33 early identification and genotyping are essential for further therapeutic treatment and the control of 34 rapid expansion of MRSA.



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